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how to choose precision casa sperm semen counting chamber slide for computer assisted sperm semen analysis software system

Choosing a precision CASA sperm semen counting chamber slide for your Computer-Assisted Sperm Analysis (CASA) system is critical to ensure accurate, reproducible, and efficient semen evaluation. The right chamber slide must be fully compatible with your CASA system’s optical, software, and analytical parameters.

Here is a step-by-step guide on how to choose the right CASA-compatible counting chamber slide:


1. Match the Chamber Depth with Software Settings

  • Common chamber depths:

    • 10 µm (commonly used for CASA)

    • 20 µm (used in some manual methods)

  • The depth directly affects sperm concentration calculations.

    • CASA software needs the exact depth input to accurately calculate sperm concentration (cells/mL).


Check your CASA software settings — it must match the slide’s actual depth (e.g., if the slide is 20 µm, software must be set to 20 µm).


 2. Select Based on CASA System Compatibility

Different CASA systems may work best with specific chamber slides due to:

  • Optical design

  • Slide dimensions

  • Autofocus and tracking capabilities

Examples of common pairings:

CASA Brand/System Compatible Slide Types
IVOS II / CEROS II (Hamilton Thorne) Leja, Makler, CellVision, Microptic

SpermVision (Minitube) Leja or Minitube slides
AndroVision (Minitube) Minitube counting chambers
IVOS II Zoo Leja Zoo slides or Makler


Check the manufacturer's user manual or contact the supplier for certified slide compatibility.


3. Choose Slide Type: Disposable vs. Reusable

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